Composite

Part:BBa_K1885123:Design

Designed by: Kien Patrick Malarney   Group: iGEM16_ASIJ_Tokyo   (2016-10-29)


Novel osmY-PETase fusion protein, A0.33


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1543
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 1543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1543
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1543
    Illegal NgoMIV site found at 818
    Illegal NgoMIV site found at 872
    Illegal NgoMIV site found at 899
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added the glycine-serine linker because we considered the c-terminal osmY domains and n-terminal PETase domains interfering with one another's folding.


Source

The osmY and PETase sequences were drawn from genome sequences of escherichia coli and ideonella sakanesis, respectively.

References